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beads sepharose cl 4b  (Bio-Rad)


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    Structured Review

    Bio-Rad beads sepharose cl 4b
    Beads Sepharose Cl 4b, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beads+sepharose+cl+4b/Econo-Pac+Chromatography+Columns/pm41131661-75-19-28
    Average 96 stars, based on 843 article reviews
    beads sepharose cl 4b - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Magnetic Induction Heating Enables On-Demand Drug Release via Diels–Alder Polymeric Nanocarriers
    Article Snippet: .. The mNPs in the filtrate were purified via size exclusion chromatography (SEC) performed with SEC beads (Sepharose CL-4B, Cytiva, Tokyo, Japan) by gravity chromatography with Econo-Pac chromatography columns (Bio-Rad Laboratories, Inc., CA, US). .. Each 0.5 mL of eluted mNP fraction was collected using a 1.5 mL Eppendorf tube and measured each fraction component at 310 (CTA) and 500 (mNPs) nm by a NanoDrop One c (Thermo Fisher Scientific, Inc., MA, US).

    Article Title: Magnetic Induction Heating Enables On-Demand Drug Release via Diels-Alder Polymeric Nanocarriers.
    Article Snippet: .. The mNPs in the filtrate were purified via size exclusion chromatography (SEC) performed with SEC beads (Sepharose CL-4B, Cytiva, Tokyo, Japan) by gravity chromatography with Econo-Pac chromatography columns (Bio-Rad Laboratories, Inc., CA, US). .. Each 0.5 mL of eluted mNP fraction was collected using a 1.5 mL Eppendorf tube and measured each fraction component at 310 (CTA) and 500 (mNPs) nm by a NanoDrop Onec (Thermo Fisher Scientific, Inc., MA, US).

    Size-exclusion Chromatography:

    Article Title: Magnetic Induction Heating Enables On-Demand Drug Release via Diels–Alder Polymeric Nanocarriers
    Article Snippet: .. The mNPs in the filtrate were purified via size exclusion chromatography (SEC) performed with SEC beads (Sepharose CL-4B, Cytiva, Tokyo, Japan) by gravity chromatography with Econo-Pac chromatography columns (Bio-Rad Laboratories, Inc., CA, US). .. Each 0.5 mL of eluted mNP fraction was collected using a 1.5 mL Eppendorf tube and measured each fraction component at 310 (CTA) and 500 (mNPs) nm by a NanoDrop One c (Thermo Fisher Scientific, Inc., MA, US).

    Article Title: Magnetic Induction Heating Enables On-Demand Drug Release via Diels-Alder Polymeric Nanocarriers.
    Article Snippet: .. The mNPs in the filtrate were purified via size exclusion chromatography (SEC) performed with SEC beads (Sepharose CL-4B, Cytiva, Tokyo, Japan) by gravity chromatography with Econo-Pac chromatography columns (Bio-Rad Laboratories, Inc., CA, US). .. Each 0.5 mL of eluted mNP fraction was collected using a 1.5 mL Eppendorf tube and measured each fraction component at 310 (CTA) and 500 (mNPs) nm by a NanoDrop Onec (Thermo Fisher Scientific, Inc., MA, US).

    Chromatography:

    Article Title: Magnetic Induction Heating Enables On-Demand Drug Release via Diels–Alder Polymeric Nanocarriers
    Article Snippet: .. The mNPs in the filtrate were purified via size exclusion chromatography (SEC) performed with SEC beads (Sepharose CL-4B, Cytiva, Tokyo, Japan) by gravity chromatography with Econo-Pac chromatography columns (Bio-Rad Laboratories, Inc., CA, US). .. Each 0.5 mL of eluted mNP fraction was collected using a 1.5 mL Eppendorf tube and measured each fraction component at 310 (CTA) and 500 (mNPs) nm by a NanoDrop One c (Thermo Fisher Scientific, Inc., MA, US).

    Article Title: Magnetic Induction Heating Enables On-Demand Drug Release via Diels-Alder Polymeric Nanocarriers.
    Article Snippet: .. The mNPs in the filtrate were purified via size exclusion chromatography (SEC) performed with SEC beads (Sepharose CL-4B, Cytiva, Tokyo, Japan) by gravity chromatography with Econo-Pac chromatography columns (Bio-Rad Laboratories, Inc., CA, US). .. Each 0.5 mL of eluted mNP fraction was collected using a 1.5 mL Eppendorf tube and measured each fraction component at 310 (CTA) and 500 (mNPs) nm by a NanoDrop Onec (Thermo Fisher Scientific, Inc., MA, US).



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    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).
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    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).
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    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).
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    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).
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    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).
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    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).
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    Image Search Results


    (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).

    Journal: Life Science Alliance

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs

    doi: 10.26508/lsa.202503341

    Figure Lengend Snippet: (A, B) miR-29a-3p and miR-1207-5p overexpression down-regulate PARN mRNA and PARN protein. (A) qRT-PCR Relative Quantitive Chart representing PARN mRNA levels upon miR-29a-3p (blue columns) or miR-1207-5p (pink columns) overexpression. (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns (24, 48) indicate hours post transfection. (B) Western blot showing PARN protein levels upon miR-29a-3p and miR-1207-5p overexpression 48 and 72 h post transfection. Extracts from WT (lane 1) and empty-vectortransfected H520 cells (lane 2) served as controls. β-actin was used as a loading reference. (C, D) miR-29a-3p and miR-1207-5p inhibition up-regulates PARN. (C) PARN mRNA levels in the presence of miR-29a-3p or miR-1207-5p LNAs in NCI-H520 cells. Cells were transfected with LNA against miR-29a-3p (blue columns) or LNA against miR-1207-5p (pink bars) and PARN mRNA levels were measured with qRT-PCR. A scramble LNA was used as a negative control (gray bars). (C) Green bar indicates PARN mRNA levels from WT (not transfected) cells used for normalization. Numbers below the columns indicate hours post transfection. (D) Levels of PARN protein upon transfection with LNAs against miR-29a-3p or miR-1207-5p. Numbers (48 and 72) above the lanes indicate hours post transfection. The type of LNAs is indicated above numbers: scramble, lanes 1 and 2; miR-29a-3p, lanes 3 and 4; miR-1207-5p, lanes 5 and 6. The red arrowhead indicates the position of 74-kD PARN (B, D).

    Article Snippet: The extracts were precleared with 50 μl of protein A-Sepharose CL-4B beads (Acris Antibodies) at 4°C for 1 h with rotation followed by centrifugation at 10,000 g in 4°C for 10 min.

    Techniques: Over Expression, Quantitative RT-PCR, Transfection, Western Blot, Inhibition, Negative Control